Cytochrome P450 Protocols

Author: Ian R. Phillips
Editor: Springer Science & Business Media
ISBN: 1592599982
File Size: 62,84 MB
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For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
Cytochrome P450 Protocols
Language: en
Pages: 364
Authors: Ian R. Phillips
Categories: Science
Type: BOOK - Published: 2008-02-04 - Publisher: Springer Science & Business Media

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of
Cytochrome P450
Language: en
Pages: 912
Authors: Paul R. Ortiz de Montellano
Categories: Medical
Type: BOOK - Published: 2015-03-13 - Publisher: Springer

This authoritative Fourth Edition summarizes the advances of the past decade concerning the structure, mechanism, and biochemistry of cytochrome P450 enzymes, with sufficient coverage of earlier work to make each chapter a comprehensive review of the field. Thirteen chapters are divided into two detailed volumes, the first covering the fundamentals
Cytochrome P450
Language: en
Pages: 461
Authors: Eric F. Johnson, Michael R. Waterman
Categories: Biochemistry
Type: BOOK - Published: 2002 - Publisher: Academic Press

Human genomics and genetics; Structure and mechanism; Regulation of expression; Metabolism; Invertebrate P450s.
Cytochrome P450 2D6
Language: en
Pages: 508
Authors: Shufeng Zhou
Categories: Medical
Type: BOOK - Published: 2018-09-03 - Publisher: CRC Press

Cytochromes are proteins that catalyze electron transfer reactions of well-known metabolic pathways and are classified in various superfamilies. The CYP, or P450, superfamily accounts for 90% of the oxidative metabolism of clinical drugs. One member of this superfamily, P450 2D6 (or CYP2D6), singlehandedly metabolizes about 25% of all medications in
Cytochrome P450
Language: en
Pages: 421
Authors: Eric F. Johnson, Michael R. Waterman
Categories: Science
Type: BOOK - Published: 2002-11-07 - Publisher: Elsevier

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all