Neutrophil Methods And Protocols

Author: Mark T. Quinn
Editor: Springer Science & Business Media
ISBN: 1588297888
File Size: 57,96 MB
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This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.

Neutrophil

Author: Mark T. Quinn
Editor: Humana
ISBN: 9781071601563
File Size: 54,79 MB
Format: PDF, ePub
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Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils. Features • Provides neutrophil protocols that are broadly applicable to leukocyte biology • Includes protocols for isolation of neutrophils from humans and animal species • Presents an interesting and informative overview of the field of neutrophil biology • Facilitates a better understanding of the role of neutrophils in host defense Readership Molecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists

Gene Therapy Protocols

Author: Joseph LeDoux
Editor: Humana Press
ISBN: 9781603272476
File Size: 68,44 MB
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In Gene Therapy Protocols, Volumes 1 and 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). In this second volume, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors.

Bioinformatics

Author: Jonathan M. Keith
Editor: Humana Press
ISBN: 9781603274289
File Size: 17,62 MB
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In Bioinformatics, leading researchers provide a selection of the most useful and widely applicable methods, able to be applied as is, or with minor variations, to many specific problems. Volume II: Structure, Function and Applications contains methods pertinent to the prediction of protein and RNA structures and the analysis and classification of structures, methods for inferring the function of previously identified genomic elements, chiefly protein-coding genes, medical applications in diagnostics and drug discovery, and "meta-methods" for developers of bioinformatics algorithms. Over 80 authors from around the globe have contributed to the two volumes.

2d Page

Author: Anton Posch
Editor:
ISBN:
File Size: 41,27 MB
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2d Page Sample Preparation And Fractionation

Author: Anton Posch
Editor: Humana Press
ISBN: 9781603272094
File Size: 41,71 MB
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Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.

Cell Cell Interactions

Author: Sean P. Colgan
Editor: Springer Science & Business Media
ISBN: 1597451134
File Size: 52,15 MB
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A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Cytoskeleton Methods And Protocols

Author: Ray H. Gavin
Editor: Humana
ISBN:
File Size: 58,92 MB
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In this cutting-edge book, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research. There is emphasis on animal, plant, protist, and fungal model systems.

Tumor Immunology And Immunotherapy Cellular Methods Part B

Author:
Editor: Academic Press
ISBN: 0128186755
File Size: 57,31 MB
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Tumor Immunology and Immunotherapy - Cellular Methods Part B, Volume 632, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered include Quantitation of calreticulin exposure associated with immunogenic cell death, Side-by-side comparisons of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death, Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry, Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies, Methods to assess DC-dependent priming of T cell responses by dying cells, and more. Contains content written by authorities in the field Provides a comprehensive view on the topics covered Includes a high level of detail

Inflammatory Mediators Activated Neutrophils And Bronchospasm In Equine Small Airways

Author: Michal Adam Olszewski
Editor:
ISBN:
File Size: 63,47 MB
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Immunocytochemical

Author: Lorette C. Javois
Editor: Springer Science & Business Media
ISBN: 1592592139
File Size: 80,72 MB
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Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.

The Journal Of Immunology

Author:
Editor:
ISBN:
File Size: 34,53 MB
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The Influence Of Pasteurella Haemolytica And Its Products On Bovine Neutrophil Function

Author: Gary Lee Watson
Editor:
ISBN:
File Size: 38,54 MB
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Current Protocols In Immunology

Author: John E. Coligan
Editor: Current Protocols
ISBN:
File Size: 17,56 MB
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Current Protocols in Immunology is a three-volume looseleaf manual that provides comprehensive coverage of immunological methods from classic to the most cutting edge, including antibody detection and preparation, assays for functional activities of mouse and human cells involved in immune responses, assays for cytokines and their receptors, isolation and analysis of proteins and peptides, biochemistry of cell activation, molecular immunology, and animal models of autoimmune and inflammatory diseases. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Bimonthly updates, which are filed into the looseleaf, keep the set current with the latest developments in immunology methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Human Genetics, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.

Adhesion And Signaling Events In Selectin And Beta 2 Integrin Mediated Neutrophil Recruitment

Author: Chad Edward Green
Editor:
ISBN:
File Size: 42,85 MB
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American Journal Of Respiratory And Critical Care Medicine

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Editor:
ISBN:
File Size: 48,56 MB
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Causal Roles For Neutrophils And Glutathione In The Liver Injury Caused By Alpha Naphthylisothiocyanate

Author: Lawrence J. Dahm
Editor:
ISBN:
File Size: 21,49 MB
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Biophysical Analysis Of Neutrophil Motility

Author: Scott Simon
Editor:
ISBN:
File Size: 41,92 MB
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Tissue Engineering Methods And Protocols

Author: Jeffrey R. Morgan
Editor: Springer Science & Business Media
ISBN: 0896035166
File Size: 27,47 MB
Format: PDF, Mobi
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In recent years, the field of tissue engineering has begun, in part, to c- lesce around the important clinical goal of developing substitutes or repla- ments for defective tissues or organs. These efforts are focused on many tissues including skin, cartilage, liver, pancreas, bone, blood, muscle, the vascu- ture, and nerves. There is a staggering medical need for new and effective treatments for acquired as well as inherited defects of organs/tissues. Tissue engineering is at the interface of the life sciences, engineering, and clinical medicine and so draws upon advances in cell and molecular biology, mate- als sciences, and surgery, as well as chemical and mechanical engineering. Such an interdisciplinary field requires a broad knowledge base as well as the use of a wide assortment of methods and approaches. It is hoped that by bringing together these protocols, this book will help to form connections - tween the different disciplines and further stimulate the synergism underlying the foundation of the tissue engineering field.

Immunocytochemical Methods And Protocols

Author: Lorette C. Javois
Editor: Springer Science & Business Media
ISBN: 1592595219
File Size: 77,24 MB
Format: PDF
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Lorette Javois' timely new 2nd edition of Immunocytochemistry Methods revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization. From Reviews of the First Edition: a useful, sometimes beautiful compilation of protocols and methods - FEBS Letters an extremely useful vade mecum' of comprehensive, detailed recipes, good advice and helpful information, spiral bound in a handy size for laboratory use.