Pcr Cloning Protocols

Author: Bing-Yuan Chen
Editor: Springer Science & Business Media
ISBN: 1592591779
File Size: 23,95 MB
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Annotation PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Pcr Cloning Protocols

Author: Bruce Alan White
Editor: Springer
ISBN: 1592595537
File Size: 62,27 MB
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Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.

Gene Isolation And Mapping Protocols

Author: Jacqueline Boultwood
Editor: Springer Science & Business Media
ISBN: 1592595545
File Size: 10,11 MB
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An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.

Pcr Methods And Applications

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ISBN:
File Size: 52,59 MB
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Current Protocols In Molecular Biology

Author: Frederick M. Ausubel
Editor:
ISBN:
File Size: 16,19 MB
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Practical Protocols In Molecular Biology

Author: Yongming Li
Editor: Science Press (NY)
ISBN: 9787030050779
File Size: 42,92 MB
Format: PDF, ePub
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Basic Dna And Rna Protocols

Author: Adrian J. Harwood
Editor: Humana PressInc
ISBN:
File Size: 55,25 MB
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An essential core collection of the latest molecular and genetic techniques for cloning subcloning sequencing PCR protein expression and much more. Each protocol represents a time-tested step-by-step recipe that creates an understanding of the procedure easily reproducible results and confidence that the procedure will work. The collection includes not only many updated and improved classic techniques but also a powerful group of advanced methods that point to future progress among them nonisotopic DNA labeling silver staining and automatic sequencing. This excellent bench companion will help those who need to learn for the first time how to conduct research on the molecular biology of nucleic acids or those who need to broaden their competence and laboratory skills. Even highly skilled researchers will find many time-saving techniques.

Biochemicals Reagents Kits For Life Science Research

Author: Sigma Chemical Company
Editor:
ISBN:
File Size: 69,68 MB
Format: PDF
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Caryologia

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ISBN:
File Size: 49,55 MB
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Report Of The Council For Tobacco Research U S A Inc

Author: Council for Tobacco Research--U.S.A.
Editor:
ISBN:
File Size: 17,38 MB
Format: PDF
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Biochemicals And Reagents For Life Science Research

Author: Sigma Chemical Company
Editor:
ISBN:
File Size: 11,20 MB
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Chemtracts

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ISBN:
File Size: 76,28 MB
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Consists of reviews, condensations, and commentaries.

Environmental Health Perspectives

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ISBN:
File Size: 21,92 MB
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Proceedings Of The National Academy Of Sciences Of The United States Of America

Author: National Academy of Sciences (U.S.)
Editor:
ISBN:
File Size: 17,45 MB
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Emerging Infectious Diseases

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ISBN:
File Size: 55,39 MB
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Plant Virology Protocols

Author: Gary Foster
Editor: Humana Press
ISBN: 9781588298270
File Size: 57,89 MB
Format: PDF, Docs
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Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section presents the most novel methods and step-by-step reproducible laboratory protocols to allow researchers more effective approaches to study plant viruses. This updated book will prove indispensable to laboratory investigators studying plant viruses.

Pcr Protocols In Molecular Toxicology

Author: John P. Vanden Heuvel
Editor: CRC Press
ISBN: 9781439805800
File Size: 24,64 MB
Format: PDF, ePub
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Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methods PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.