Perfect Targets

Author: Rebekah Heinrichs
Editor: AAPC Publishing
ISBN: 9781931282185
Size: 16,99 MB
Format: PDF, Mobi
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A 2002 study from Comprehensive Issues in Pediatric Nursing found that 94 percent of students with Asperger Syndrome face torment from their peers. Indeed, some of their behaviors and characteristics that others see as "different" make many of these children easy targets for frequent and severe bullying. This book takes a frank look at the different types of bullying and what adults must do to curb bullying, helping prevent the often lifelong effects of this behavior on its victims. Practical strategies and solutions at the school, class and individual level are presented.

Perfect Target

Author: Stephanie Newton
Editor: Harlequin Books
ISBN: 9780373443321
Size: 19,48 MB
Format: PDF
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A corpse in her path and a break-in at home warn Bayley Foster that the stalker who once tormented her has returned. Her fiercely protective neighbor, police detective Cruse Conyers, is determined to get answers, even if it takes him deep into the criminal underworld. Original.

A Role For The Let 7 Primary Microrna In Target Gene Recognition And Repression

Author:
Editor: Stanford University
ISBN:
Size: 19,38 MB
Format: PDF, Mobi
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MicroRNA (miRNA) genes produce three noncoding RNA products: the long primary transcript (pri-miRNA), the ~70 nucleotide pre-miRNA, and the ~22-nt mature miRNA. Only the mature miRNA is considered to be the functional species of a miRNA gene in recognizing cognate target mRNAs and modulating their expression. However, mature miRNAs are processed from the primary transcript through sequential endonucleolytic steps. As a result, the mature miRNA sequence is present in all three RNA products of a miRNA gene. It has thus been intrinsically difficult to determine the contribution of each miRNA gene product to target repression. In fact, direct functional roles for pri- and pre-miRNAs have never been ruled out. Here we show that pri- and pre-miRNAs may not be mere transitory intermediates of mature miRNA biogenesis. We found that ectopic expression of the C. elegans miRNA gene let-7 (cel-let-7) in human culture cells results in the production of truncated pre- and mature miRNAs that lack the first two 5' nucleotides, one of which is the first nucleotide of the miRNA seed region (nucleotide SD1). We found this nucleotide to be required for repression of target reporters by cel-let-7 in these cells, demonstrating that pri-let-7 may have a direct role in target repression. Further, we show that the nucleotide sequence and structure of both the pri-/pre-let-7 loop and stem regions play a key role in miRNA gene function in reporter assays. In vitro and in vivo analyses indicated the significance of these regions may be in the mediation of a physical interaction between pri-let-7 and target RNAs. These observations suggest that regulatory information encoded in the structured pri-miRNAs, but absent from mature miRNAs, could be directly interpreted for target recognition and repression through RNA:RNA interaction. Intriguingly, some mutations in the loop nucleotide sequence also restored processing of the 5' ends of C. elegans pre- and mature let-7 in culture cells, demonstrating that the pri-/pre-miRNA loop region can also regulate the precision of mature miRNA biogenesis. Importantly, in the presence of functional pre- and mature let-7, cel-let-7 activity in target repression consists of both SD1-independent and SD1-dependent components, implying potential contributions by both pri- and mature let-7. Finally, we interrogated the effects of pri-/pre-let-7 loop mutations on their ability to rescue a let-7 loss-of-function mutant phenotype in C. elegans. Our results indicate decreased significance of these parameters in the control of worm vulval development, although context-dependent differences in mature miRNA biogenesis between heterologous culture and live animals may partially explain this discrepancy. Taken together the work presented here reveals a novel layer of regulatory complexity encoded in long primary miRNAs that may have broad implications in understanding the mechanisms by which miRNA genes control target expression.

Molecular Beacons

Author: Chaoyong James Yang
Editor: Springer Science & Business Media
ISBN: 3642391095
Size: 16,18 MB
Format: PDF, ePub, Docs
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Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.

Dusted Horsemen

Author: Richard Dempsey
Editor: Xlibris Corporation
ISBN: 1413495192
Size: 17,62 MB
Format: PDF
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Poetry has power, and Wm. Richard Dempsey proves it again and again in this volume of verse, perspective, and meditations. Here are a fresh voice, accessible vision, clear images. Historian and retired intelligence officer, poet and novelist, he offers a questing intellect, a sensitive ear, and a gentle, sure pen. ""I want to write poems that ordinary people would read and understand,"" the author says. ""At the same time, I like to deal with important questions of our age. I do not shy away from hard issues."" His poems and meditations deal with love and loneliness, with reflections on the cosmic mystery, with 9/11 and the Holocaust, and with the human thirst for understanding. This is an important book written in clear language by a writer attuned to our time.

Soft Target Hardening

Author: Jennifer Hesterman
Editor: CRC Press
ISBN: 1482244225
Size: 10,40 MB
Format: PDF, ePub, Mobi
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Winner of ASIS Internationals 2015 Security Book of the Year AwardTerrorist attacks occur daily and are increasingly being aimed at civilian populations around the world. Battlefield lines have been redrawn to include churches, schools, hospitals, and malls. This breach of moral boundary is shocking and invokes feartwo primary goals of terrorism.

The Redwood Stumper 2009

Author: Paul Feist
Editor: Paul Feist
ISBN: 1300035722
Size: 20,28 MB
Format: PDF, ePub
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The Newsletter of the Redwood Gun Club in Humboldt County California. A newsletter dedicated to sharing the activities, interests, and knowledge of the membership of the club with our community. This is every issue of the "Redwood Stumper" from 2009

Therapeutic Monoclonal Antibodies

Author: Zhiqiang An
Editor: John Wiley & Sons
ISBN: 1118210263
Size: 14,89 MB
Format: PDF, ePub, Mobi
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70-chapter authoritative reference that covers therapeutic monoclonal antibody discovery, development, and clinical applications while incorporating principles, experimental data, and methodologies. First book to address the discovery and development of antibody therapeutics in their entirety. Most chapters contain experimental data to illustrate the principles described in them. Authors provide detailed methodologies that readers can take away with them and use in their own laboratories.

Radar Imaging Of Airborne Targets

Author: Brett Borden
Editor: CRC Press
ISBN: 9781420069006
Size: 15,69 MB
Format: PDF, Kindle
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Radar-based imaging of aircraft targets is a topic that continues to attract a lot of attention, particularly since these imaging methods have been recognized to be the foundation of any successful all-weather non-cooperative target identification technique. Traditional books in this area look at the topic from a radar engineering point of view. Consequently, the basic issues associated with model error and image interpretation are usually not addressed in any substantive fashion. Moreover, applied mathematicians frequently find it difficult to read the radar engineering literature because it is jargon-laden and device specific, meaning that the skills most applicable to the problem's solution are rarely applied. Enabling an understanding of the subject and its current mathematical research issues, Radar Imaging of Airborne Targets: A Primer for Applied Mathematicians and Physicists presents the issues and techniques associated with radar imaging from a mathematical point of view rather than from an instrumentation perspective. The book concentrates on scattering issues, the inverse scattering problem, and the approximations that are usually made by practical algorithm developers. The author also explains the consequences of these approximations to the resultant radar image and its interpretation, and examines methods for reducing model-based error.

The Accidental President

Author: Harris I. Baseman
Editor: iUniverse
ISBN: 0595755151
Size: 20,19 MB
Format: PDF
Read: 416
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When an explosion rips through the home of Gulf War veteran Clarence Davenport, Secret Service Agent John Wallace and White House aide Molly Pemberton are certain that Davenport, the assassin who killed President Butler, had killed himself. President Silver, the former Education Secretary who became President when Davenport killed all other legal successors to the Presidency, informs the nation that the crime of the century has been solved and that the perpetrator is dead, only to have to admit, days later, that Davenport had escaped. National outrage at Davenport's escape escalates, fueled by Senator Jeb Davies' political ambition and his hatred of Ben Silver. Davenport, the world's most sought after fugitive, with no other place to go, is recruited by Saudi expatriates opposed to the Royal Family and ends up in an Al Qaeda training camp where he devises another plan to kill President Silver and attack the United States. Silver tries to stave off the rising tide of impeachment and at the same time combat the increasing risk of a terrorist attack that will destroy the Presidency and fundamentally change the Unites States unless he can avoid impeachment and intercept the threat. " timely as this evening's news " -Melannie Lauers, Cape Cod Times(on After Kamisiyah) " an intriguing insight " -Mathew Call, Newton Tab (on After Kamisiyah)