Second Harmonic Generation Imaging

Author: Francesco S. Pavone
Editor: Taylor & Francis
ISBN: 1439849153
File Size: 78,83 MB
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Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical applications. The book features contributions by experts in second-harmonic imaging, including many pioneering researchers in the field. Written for researchers at all levels, it takes an in-depth look at the current state of the art and possibilities of SHG microscopy. Organized into three sections, the book: Provides an introduction to the physics of the process, step-by-step instructions on how to build an SHG microscope, and comparisons with related imaging techniques Gives an overview of the capabilities of SHG microscopy for imaging tissues and cells—including cell membranes, muscle, collagen in tissues, and microtubules in live cells—by summarizing experimental and analytical methods Highlights representative biomedical and medical applications in imaging cancer, fibroses, autoimmune diseases, connective tissue disorders, eye pathologies, and cardiovascular disease Historically, clinical imaging at the cellular and tissue level has been performed by pathologists on ex vivo biopsies removed by the surgeon. While histology remains the "gold standard" for pathologists, its interpretation remains highly subjective. Much of SHG research has focused on developing more quantitative, objective metrics. A tutorial for newcomers and an up-to-date review for experts, this book explores how SHG may be used to more precisely image a wide range of pathological conditions and diseases.

Second Harmonic Generation Imaging

Author: Francesco Pavone
Editor:
ISBN:
File Size: 33,73 MB
Format: PDF
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Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a.

Second Harmonic Generation Imaging Of A Magnetic Topological Insulator

Author: Carina Belvin
Editor:
ISBN:
File Size: 38,72 MB
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A topological insulator (TI) is a type of quantum material that is insulating in the bulk but metallic on the surface. Due to the unique spin properties of the surface electrons, TIs have attracted much interest for their potential applications in spin-based electronics and quantum computers. Even more exotic effects occur when TIs are brought in contact with magnetic materials. This thesis represents a study of two-layer thin films consisting of the TI Bi2Se3 and the magnetic insulator EuS using a nonlinear optical technique called second harmonic generation (SHG) imaging. SHG imaging can probe the crystal and magnetic structure at the surfaces and interfaces of inversion symmetric materials. At the interface between the EuS and Bi2Se3 layers, magnetic domains are expected to form. The domain boundaries are predicted to host chiral edge states, which are dissipationless currents that flow in one direction around a domain. Using SHG imaging, our goal was to visualize the magnetic domains in this magnetic topological insulator system. Even though we have yet to observe any evidence of magnetic domains in this material, we also performed SHG imaging on monolayer MoS2 and were able to visualize distinct crystal grains. Our SHG imaging setup that was improved upon during this thesis has the potential to reveal information about other interesting quantum materials.

Identification Of Quantitative Second Harmonic Generation Imaging Metrics For Collagenous Tissue

Author: Woowon Lee
Editor:
ISBN:
File Size: 25,34 MB
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Multispectral Imaging And Second Harmonic Generation In Scanning Laser Optical Tomography

Author: Lena Nolte
Editor:
ISBN: 9783959003384
File Size: 28,92 MB
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Optics And Lasers In Biomedicine And Culture

Author: Costas Fotakis
Editor: Springer Science & Business Media
ISBN: 9783540666486
File Size: 43,98 MB
Format: PDF, Mobi
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Following to previous OWLS conferences devoted to widespread applications of optics in life sciences, this 5th OWLS Conference focuses on recent achievements in applying lasers and optics in biomedicine and the preservation of our cultural heritage. Particular attention is paid to laser diagnostics in medicine, interaction of laser radiation with biological tissue, aspects of the preservation of cultural heritage, and the development of new systems for these studies. The contributors to this volume cover international research activities in the following areas: Laser-tissue interactions and tissue optics - photon migration in tissue; Medical sensors - fiber optics; Clinical use of lasers (dermatology, ENT, cardiology, etc.); Laser-based techniques in art conservation (cleaning, diagnostics, analytical applications); Imaging techniques and lasers in archaeology; Laser technologies in contemporary art (holography, marking, etc.); and New laser and opto-electronic systems for biomedical and art-related studies.

Polarization Modulated Second Harmonic Generation Microscopy In Collagen

Author: Patrick C. Stoller
Editor:
ISBN:
File Size: 58,13 MB
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Imaging Manipulation And Analysis Of Biomolecules Cells And Tissues

Author:
Editor:
ISBN:
File Size: 46,82 MB
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Nanoscopy And Multidimensional Optical Fluorescence Microscopy

Author: Alberto Diaspro
Editor: CRC Press
ISBN: 1420078895
File Size: 79,98 MB
Format: PDF, Kindle
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"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.

Investigation And Imaging Of Noncentrosymmetric Macromolecules In Biological Systems

Author: Mingshi Wang
Editor:
ISBN:
File Size: 15,30 MB
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Advanced Biophotonics

Author: Ruikang K. Wang
Editor: CRC Press
ISBN: 1439895813
File Size: 48,58 MB
Format: PDF
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Despite a number of books on biophotonics imaging for medical diagnostics and therapy, the field still lacks a comprehensive imaging book that describes state-of-the-art biophotonics imaging approaches intensively developed in recent years. Addressing this shortfall, Advanced Biophotonics: Tissue Optical Sectioning presents contemporary methods and applications of biophotonics imaging. Gathering research otherwise scattered in numerous physical, chemical, biophysical, and biomedical journals, the book helps researchers, bioengineers, and medical doctors understand major recent bioimaging technologies and the underlying biophotonics science. Well-known international experts explore a variety of "hot" biomedical optics and biophotonics problems, including the use of photoacoustic imaging to investigate the molecular and cellular processes in living systems. The book also covers Monte Carlo modeling, tissue optics and tissue optical clearing, nonlinear optical microscopy, various aspects of optical coherence tomography, multimodal tomography, adaptive optics, and signal imaging. With 58 color images, this book represents a valuable contribution to the biomedical and biophotonics literature. Designed for researchers and practitioners in biophotonics, the book is also a useful resource for scientists in laser physics and technology, fiber optics, spectroscopy, materials science, biology, and medicine as well as students studying biomedical physics and engineering, biomedical optics, and biophotonics.

Optical Fluorescence Microscopy

Author: Alberto Diaspro
Editor: Springer Science & Business Media
ISBN: 9783642151750
File Size: 56,29 MB
Format: PDF
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In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Quantitative Imaging In Cell Biology

Author:
Editor: Academic Press
ISBN: 0124202012
File Size: 18,93 MB
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This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Optical Sensing Imaging And Manipulation For Biological And Biomedical Applications

Author: Society of Photo-optical Instrumentation Engineers
Editor: Society of Photo Optical
ISBN:
File Size: 74,46 MB
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Multiphoton Microscopy In The Biomedical Sciences

Author:
Editor:
ISBN:
File Size: 53,72 MB
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Commercial And Biomedical Applications Of Ultrafast And Free Electron Lasers

Author: Glenn S. Edwards
Editor: Society of Photo Optical
ISBN:
File Size: 78,43 MB
Format: PDF, ePub
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Optics In Tissue Engineering And Regenerative Medicine

Author: Sean Kirkpatrick
Editor: Society of Photo Optical
ISBN:
File Size: 46,39 MB
Format: PDF, Docs
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Proceedings of SPIE present the original research papers presented at SPIE conferences and other high-quality conferences in the broad-ranging fields of optics and photonics. These books provide prompt access to the latest innovations in research and technology in their respective fields. Proceedings of SPIE are among the most cited references in patent literature.