Super Resolution Microscopy

Author: Udo J. Birk
Editor: John Wiley & Sons
ISBN: 3527341331
File Size: 40,55 MB
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This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Label Free Super Resolution Microscopy

Author: Vasily Astratov
Editor: Springer Nature
ISBN: 3030217221
File Size: 19,50 MB
Format: PDF, ePub, Mobi
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This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Super Resolution Microscopy

Author: Holger Erfle
Editor: Humana Press
ISBN: 9781493972647
File Size: 25,94 MB
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This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

Super Resolution Microscopy Development For The Detection Of Nano Structures And Confocal Study Of The Structural Damage In Gut Cell Nuclei Due To Total Body Irradiation

Author: Mehedi Hasan
Editor:
ISBN:
File Size: 15,65 MB
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Optical microscopy is the oldest form of microscopy that has been visually aiding scientific research. In our research, I have reported here two such optical microscopy techniques for two different projects. In the first project, we re-developed an instrumentation of a cost-effective, high-performing, single-molecular localization super-resolution microscopy setup that breaks the diffraction limitation barrier. Then we use a stochastic image capturing technique to capture the best precision image positions of gold nanoparticles. In our second project, we apply confocal microscopy technique to image DNA molecular nanoscale structural alterations of chromatin in cell nuclei of gut tissues caused by total body irradiation (TBI). We then quantify these alterations using a light localization technique called inverse participation ratio (IPR) using the confocal micrographs of the sample. Our results show radiation causes reduction and saturation of DNA spatial mass density fluctuations that were observed for different durations of post-irradiation.

Super Resolution Microscopy Techniques In The Neurosciences

Author: Eugenio Fornasiero
Editor: Humana Press
ISBN: 9781627039826
File Size: 34,51 MB
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Super-Resolution Microscopy Techniques in the Neurosciences serves as a comprehensive description of current super-resolution techniques, including the physical principles that allowed for their development, some of the most recent neurobiological applications and selected information for the practical use of these technologies. Written for the Neuromethods series, this detailed work contains contributions from experts in the field and provides key implementation advice to ensure successful results in the lab. Authoritative and cutting-edge, Super-Resolution Microscopy Techniques in the Neurosciences is an ideal guide for researchers aiming to continue increasing the resolution in the imaging capabilities of neuroscientists and thereby changing the perspective in which cellular biology processes are understood at the nanometer scale.

Advanced Image Deconvolution Techniques For Super Resolution Microscopy

Author: Shun Qin
Editor:
ISBN:
File Size: 40,80 MB
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In last decades, super-resolution fluorescence microscopy has seen its great development. Many super-resolution techniques are developed: the non-linear super-resolution techniques such as STED, PALM/STROM have provided the power of imaging scale under 50nm; at the meantime, many linear super-resolution techniques, such as SIM and ISM are also developed for fast and low photon bleaching super-resolution imaging. In the work of this study, I focused on studying the super-resolution techniques, such as SOFI, SIM, ISM and Airy beams light-sheet microscopy, and deconvolution algorithms for thes...

Super Resolution Microscopy To Examine Cellular Uptake Of Micelle Encapsulated Quantum Dots

Author: Wesley Chiang
Editor:
ISBN:
File Size: 75,45 MB
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Plasmonics And Super Resolution Imaging

Author: Zhaowei Liu
Editor: CRC Press
ISBN: 1351797328
File Size: 51,18 MB
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Plasmonics is an emerging field mainly developed within the past two decades. Due to its unique capabilities to manipulate light at deep subwavelength scales, plasmonics has been commonly treated as the most important part of nanophotonics. Plasmonic-assisted optical microscopy techniques, especially super-resolution microscopy, have shown tremendous potential and attracted much attention. This book aims to collect cutting-edge studies in various optical imaging technologies with advanced performances that are enabled or enhanced by plasmonics. The basic working principles, development details, and potential future direction and perspectives are discussed. Edited by Zhaowei Liu, a prominent researcher in the field of super-resolution microscopy, this book will be an excellent reference for anyone in the field of nanophotonics, plasmonics, and optical microscopy.

A New Approach To 3 Dimensional Super Resolution Microscopy

Author: Marco David Howard
Editor:
ISBN:
File Size: 42,53 MB
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Super-Resolution microscopy has transformed our ability to image biological specimens at the nanoscale with high contrast and molecular specificity. However, acquiring 3-dimensional images over large volumes remains a challenge because the imaging process causes fluorophores outside the focal volume to photobleach before they can be imaged. Additionally, acquiring highly multiplexed images is challenging due to a lack of spectrally distinct fluorophores which possess the required photophysical properties for super-resolution imaging. Here I present my work which specifically addresses these issues. In chapter 2 I first discuss the importance of sample preparation for super-resolution imaging. In chapter 3 I introduce a novel labeling scheme called probe-refresh STORM (prSTORM) which enables practitioners obtain multiplexed, extended-depth super-resolution images with a single dye, and in chapter 4 I show the importance of hardware for super-resolution microscopy

Advances In Super Resolution Microscopy And Their Application To Photosynthesis

Author: Samuel Fardell Hastings Barnett
Editor:
ISBN:
File Size: 73,37 MB
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Super Resolution Microscopy Of Synaptic Proteins

Author: Sarah Aufmkolk
Editor:
ISBN:
File Size: 10,86 MB
Format: PDF, ePub
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Super Resolution Microscopy Of Plasma Membrane Receptors And Intracellular Pathogens

Author: Ralph Götz
Editor:
ISBN:
File Size: 14,82 MB
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Super Resolution Microscopy Of Integrin 5 1 Nanoscale Receptor Clustering

Author: Stephanie Maynard
Editor:
ISBN:
File Size: 77,68 MB
Format: PDF, Kindle
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Super Resolution Microscopy Of The Synaptic Active Zone

Author: Nadine Ehmann
Editor:
ISBN:
File Size: 16,59 MB
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Live Cell Super Resolution Microscopy Quanitifies An Interaction Between Influenza Hemagglutinin And Phosphatidylinositol 4 5 Bisphosphate

Author: Jaqulin Nicole Wallace
Editor:
ISBN:
File Size: 74,13 MB
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Fluorescence Microscopy

Author: Anda Cornea
Editor: Elsevier
ISBN: 0124167136
File Size: 17,80 MB
Format: PDF
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Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Advances In Dna Paint Super Resolution Microscopy

Author: Maximilian Strauß
Editor:
ISBN:
File Size: 14,33 MB
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Single Molecule Science

Author: Krishnarao Appasani
Editor: Cambridge University Press
ISBN: 9781108423366
File Size: 18,64 MB
Format: PDF, Kindle
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Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, biological imaging, genomics and structural biology.